3V4L
Mouse MALT1(caspase-IG3 domains) in complex with a irreversible peptidic inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 150.861, 150.861, 91.822 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.290 - 3.150 |
| R-factor | 0.1808 |
| Rwork | 0.178 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.280 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 10950 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 98.5 | 97.5 |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 0.2 M Li2SO4, 0.1M Bis-tris propane pH7, pH 7.0, VAPOR DIFFUSION, temperature 298K |
