3V3K
Human caspase 9 in complex with bacterial effector protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0015 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.703, 209.906, 317.231 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 174.080 - 3.494 |
| R-factor | 0.23963 |
| Rwork | 0.239 |
| R-free | 0.25949 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.932 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 174.080 | 3.770 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.126 | 0.581 |
| Number of reflections | 86121 | |
| <I/σ(I)> | 5.9 | 1.3 |
| Completeness [%] | 98.8 | 98.3 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.75 K-Na-Tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
