3V0E
Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 256-576(C363S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1158 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 50.849, 38.605, 89.832 |
| Unit cell angles | 90.00, 103.99, 90.00 |
Refinement procedure
| Resolution | 87.170 - 1.650 |
| R-factor | 0.17829 |
| Rwork | 0.177 |
| R-free | 0.20959 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d5r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.081 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.672 |
| Number of reflections | 43750 | |
| <I/σ(I)> | 18.1 | 2.4 |
| Completeness [%] | 97.7 | 82.2 |
| Redundancy | 5.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 2.1-2.3 M ammonium sulfate, 0.1 M HEPES, pH 7.0-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
