3V0D
Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1158 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.967, 85.706, 84.327 |
| Unit cell angles | 90.00, 90.96, 90.00 |
Refinement procedure
| Resolution | 84.320 - 1.100 |
| R-factor | 0.14095 |
| Rwork | 0.140 |
| R-free | 0.16073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d5r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.472 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.054 | 0.475 |
| Number of reflections | 291443 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 4.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 17.5-22.5% PEG 2000, 0.1 M ammonium dihydrophosphate, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
