3UZE
Crystal structure of the dengue virus serotype 3 envelope protein domain III in complex with the variable domains of Mab 4E11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-06-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0721 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.055, 74.670, 86.888 |
Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
Resolution | 40.800 - 2.040 |
R-factor | 0.2185 |
Rwork | 0.218 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uzg 3uzq |
RMSD bond length | 0.007 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.9.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.857 | 42.857 | 2.150 |
High resolution limit [Å] | 2.037 | 6.440 | 2.040 |
Rmerge | 0.043 | 0.444 | |
Total number of observations | 5108 | 11687 | |
Number of reflections | 40602 | ||
<I/σ(I)> | 10.8 | 12.7 | 1.7 |
Completeness [%] | 94.8 | 95.9 | 76 |
Redundancy | 3.5 | 3.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 20% PEG 8000, 0.1M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 292K |