3UTZ
Endogenous-like inhibitory antibodies targeting activated metalloproteinase motifs show therapeutic potential
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.095, 108.369, 86.466 |
| Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
| Resolution | 41.270 - 2.180 |
| R-factor | 0.23051 |
| Rwork | 0.228 |
| R-free | 0.28789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.929 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.180 |
| Number of reflections | 72501 |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2M (NH4)2SO4, 25% PEG 4000, 15% Glycerol, 0.2M NDSB-211, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
