3UQS
Crystal structures of murine norovirus RNA-dependent RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-17 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.670, 196.330, 109.150 |
| Unit cell angles | 90.00, 114.34, 90.00 |
Refinement procedure
| Resolution | 28.750 - 2.000 |
| R-factor | 0.18832 |
| Rwork | 0.186 |
| R-free | 0.22732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.331 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.210 |
| High resolution limit [Å] | 2.000 | 2.100 |
| Number of reflections | 154866 | |
| Completeness [%] | 99.7 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 293 | 1.8M ammonium sulfate, 0.05M calcium chloride, 0.1M sodium acetate, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
