3UP3
Nuclear receptor DAF-12 from hookworm Ancylostoma ceylanicum in complex with (25S)-cholestenoic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.545, 85.296, 46.325 |
| Unit cell angles | 90.00, 106.87, 90.00 |
Refinement procedure
| Resolution | 29.290 - 1.250 |
| R-factor | 0.1672 |
| Rwork | 0.166 |
| R-free | 0.18040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.522 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.295 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 64363 | |
| Completeness [%] | 92.7 | 57.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 20% w/v PEG3350, 0.2 M diammonium tartrate, SITTING DROP, temperature 293K |
