3UM7

Crystal structure of the human two pore domain K+ ion channel TRAAK (K2P4.1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97833
Spacegroup name	P 21 21 21
Unit cell lengths	87.938, 130.852, 132.779
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.200 - 3.310
R-factor	0.3172
Rwork	0.317
R-free	0.32280
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.172
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	50.000	3.360
High resolution limit [Å]	3.300	8.940	3.300
Rmerge	0.056	0.019
Number of reflections	17761
<I/σ(I)>	11.7
Completeness [%]	73.4	86.9	2.1
Redundancy	3.7	3.3	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	21-24% PEG400, 1 mM Fos-choline-12, VAPOR DIFFUSION, HANGING DROP, temperature 277K