3UKH
Crystal structure of udp-galactopyranose mutase from Aspergillus fumigatus in complex with UDPGALP (non-reduced)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.967, 129.262, 173.885 |
| Unit cell angles | 89.87, 84.64, 81.21 |
Refinement procedure
| Resolution | 35.548 - 2.300 |
| R-factor | 0.2239 |
| Rwork | 0.222 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIALLY REFINED MODEL FROM LMUGM |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.766 |
| Data reduction software | d*TREK (9.9.8.0L) |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.548 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.069 | 0.432 |
| Number of reflections | 266596 | |
| <I/σ(I)> | 8.6 | 2.6 |
| Completeness [%] | 97.3 | 88.4 |
| Redundancy | 3.94 | 3.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 295 | 0.1M Bis-Tris-Propane pH 8.5, 0.2M NaNO2, 20% PEG 3350, microbatch, temperature 295K |
