3UIN

Complex between human RanGAP1-SUMO2, UBC9 and the IR1 domain from RanBP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	155.407, 155.407, 57.931
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.411 - 2.597
R-factor	0.2139
Rwork	0.211
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z5s
RMSD bond length	0.009
RMSD bond angle	1.176
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.690
High resolution limit [Å]	2.597	2.597
Rmerge	0.040	0.356
Number of reflections	24830
<I/σ(I)>	17.7	1.8
Completeness [%]	99.2	98
Redundancy	8.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	279	100 mM HEPES pH 7.5, 400 mM ammonium citrate, 2% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 279K