3UH2
Tankyrase-1 in complexed with PJ34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.260, 43.300, 88.540 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 62.610 - 2.000 |
| R-factor | 0.1775 |
| Rwork | 0.175 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID=2RF5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.610 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.085 | 0.415 |
| Number of reflections | 31795 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 97.2 | 96.5 |
| Redundancy | 3.61 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 298 | 16% PEG3350, 100mM Bis-Tris pH5.8, 300mM Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
