3UGX
Crystal Structure of Visual Arrestin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-14 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 168.363, 184.329, 90.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.886 - 2.649 |
| R-factor | 0.2108 |
| Rwork | 0.209 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.172 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.420 | 2.690 |
| High resolution limit [Å] | 2.649 | 2.650 |
| Rmerge | 0.075 | 0.226 |
| Number of reflections | 81412 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 98.4 | 73.8 |
| Redundancy | 5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 294.15 | 18mM PIPES,0.5M KCL,30% EG, 8% PEG 6000, 18% PEG200, 6% PEG 1000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |
