3UG3
Crystal structure of alpha-L-arabinofuranosidase from Thermotoga maritima ligand free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.515, 160.647, 155.735 |
| Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
| Resolution | 30.140 - 1.800 |
| R-factor | 0.17617 |
| Rwork | 0.175 |
| R-free | 0.19686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pz3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 446597 | |
| <I/σ(I)> | 34.1 | 3.4 |
| Completeness [%] | 97.4 | 95.6 |
| Redundancy | 7.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1M sodium citrate, 0.1M Tris-HCl, 0.2M NaCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
