3UF2

Crystal structure of the human Colony-Stimulating Factor 1 (hCSF-1) cytokine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	61.150, 104.490, 116.240
Unit cell angles	90.00, 105.29, 90.00

Refinement procedure

Resolution	24.942 - 2.750
R-factor	0.1423
Rwork	0.137
R-free	0.19030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.487
Refinement software	PHENIX ((phenix.refine: dev_874))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.000
High resolution limit [Å]	2.750	2.750
Number of reflections	36596
Completeness [%]	99.5	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1M magnesium chloride, 0.1M sodium citrate pH 5.0, 15% w/v PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K