3UEN
Crystal structure of TopBP1 BRCT4/5 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11588 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 35.902, 48.801, 126.088 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.590 - 1.900 |
| R-factor | 0.1775 |
| Rwork | 0.175 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.347 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.077 | 0.026 | 0.497 |
| Number of reflections | 18140 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 99.8 | 98.6 | 99.2 |
| Redundancy | 3.8 | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 3350, NaSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
