3UEH
Crystal structure of human Survivin H80A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.208, 71.128, 84.063 |
| Unit cell angles | 90.00, 131.43, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.1911 |
| Rwork | 0.189 |
| R-free | 0.22398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 'peb entry 3UEC |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.784 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.064 | 0.653 |
| Number of reflections | 15281 | |
| <I/σ(I)> | 35 | 2.2 |
| Completeness [%] | 97.9 | 96 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG 3350, 0.2 M SODIUM FORMATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
