3UEH
Crystal structure of human Survivin H80A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 113.208, 71.128, 84.063 |
Unit cell angles | 90.00, 131.43, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.1911 |
Rwork | 0.189 |
R-free | 0.22398 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 'peb entry 3UEC |
RMSD bond length | 0.017 |
RMSD bond angle | 1.784 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.064 | 0.653 |
Number of reflections | 15281 | |
<I/σ(I)> | 35 | 2.2 |
Completeness [%] | 97.9 | 96 |
Redundancy | 4.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG 3350, 0.2 M SODIUM FORMATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |