3UEC
Crystal structure of human Survivin bound to histone H3 phosphorylated on threonine-3.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 69.254, 70.225, 88.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.180 |
R-factor | 0.19919 |
Rwork | 0.198 |
R-free | 0.21488 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.675 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.220 |
High resolution limit [Å] | 2.180 | 2.180 |
Rmerge | 0.067 | 0.776 |
Number of reflections | 11616 | |
<I/σ(I)> | 24.2 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 12% peg 8000; O.1 M MOPS 7.5; 0.1 M Magnesium acetate tetrahydrate , VAPOR DIFFUSION, HANGING DROP, temperature 289K |