3UAJ
Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-05-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.110, 134.750, 106.080 |
| Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
| Resolution | 47.738 - 3.232 |
| R-factor | 0.2413 |
| Rwork | 0.241 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oke |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | BUSTER-TNT (BUSTER 2.9.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.738 | 47.738 | 3.410 |
| High resolution limit [Å] | 3.232 | 10.220 | 3.232 |
| Rmerge | 0.024 | 0.465 | |
| Total number of observations | 4227 | 16475 | |
| Number of reflections | 39891 | ||
| <I/σ(I)> | 10 | 27.2 | 1.7 |
| Completeness [%] | 97.3 | 96.3 | 91.7 |
| Redundancy | 3.4 | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 292 | 8% PEG8000, 8% MPD, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, temperature 292K |
