3U9Q
Ligand binding domain of PPARgamma complexed with Decanoic Acid and PGC-1a peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-06-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.726, 54.346, 66.809 |
| Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
| Resolution | 30.082 - 1.522 |
| R-factor | 0.1851 |
| Rwork | 0.183 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.654 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.082 | 1.562 |
| High resolution limit [Å] | 1.520 | 1.522 |
| Number of reflections | 43465 | |
| Completeness [%] | 94.9 | 97.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 298 | 0.2 M sodium acetate (pH 6.2), 20% PEG 3350, 15% glycerol and 1 mM concentration of the ligand., VAPOR DIFFUSION, temperature 298K |
