3U9D

Crystal Structure of a chimera containing the N-terminal domain (residues 8-24) of drosophila Ciboulot and the C-terminal domain (residues 13-44) of bovine Thymosin-beta4, bound to G-actin-ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97903
Spacegroup name	P 1 21 1
Unit cell lengths	45.698, 75.738, 128.518
Unit cell angles	90.00, 90.03, 90.00

Refinement procedure

Resolution	19.640 - 2.500
R-factor	0.20598
Rwork	0.204
R-free	0.23974
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sqk
RMSD bond length	0.012
RMSD bond angle	1.421
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (1.3.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		2.550
High resolution limit [Å]	2.500	17.000	2.500
Rmerge	0.061	0.015	0.294
Number of reflections	29916
<I/σ(I)>	17.25	40.34	4.31
Completeness [%]	97.8	32.1	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	298	18% PEG3350, 0.05M NaAcetate pH4.7, 0.1M MgAcetate pH6.5, 0.32M Guanidine HCl, 0.8% Dioxane, hanging drop, temperature 298K