3U94
Crystal structure of the GluK3 ligand binding domain complex with glutamate and zinc: P21212 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 89.155, 111.374, 129.851 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.937 - 1.962 |
R-factor | 0.196 |
Rwork | 0.193 |
R-free | 0.24680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s50 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.384 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.085 | 0.490 |
<I/σ(I)> | 26.9 | 3.7 |
Completeness [%] | 98.0 | 97.7 |
Redundancy | 8 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 6% PEG 3350, 0.1 M BisTrisPropane, 0.2 M NaSulfate, 5 mM glutamate, 10 mM ZnAcate,, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |