3U94

Crystal structure of the GluK3 ligand binding domain complex with glutamate and zinc: P21212 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	89.155, 111.374, 129.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.937 - 1.962
R-factor	0.196
Rwork	0.193
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s50
RMSD bond length	0.017
RMSD bond angle	1.384
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.990
High resolution limit [Å]	1.960	1.960
Rmerge	0.085	0.490
<I/σ(I)>	26.9	3.7
Completeness [%]	98.0	97.7
Redundancy	8	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	6% PEG 3350, 0.1 M BisTrisPropane, 0.2 M NaSulfate, 5 mM glutamate, 10 mM ZnAcate,, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K