3U93
Crystal structure of the GluK3 ligand binding domain complex with glutamate and zinc: P2221 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 130.401, 55.457, 88.861 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.046 - 1.881 |
R-factor | 0.2159 |
Rwork | 0.214 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s50 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.918 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.910 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.049 | 0.473 |
Number of reflections | 53068 | |
<I/σ(I)> | 23.8 | 2.8 |
Completeness [%] | 98.4 | 95.2 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 14% PEG 3350, 0.1 M BisTrisPropane, 0.2 M Na2SO4, 5 mM glutamate, 5 mM ZnAcetate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |