3U8U
Crystal structure of Human Apurinic/Apyridinimic Endonuclease, Ape1 in a new crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.840, 97.284, 132.150 |
| Unit cell angles | 90.00, 90.91, 90.00 |
Refinement procedure
| Resolution | 40.710 - 2.150 |
| R-factor | 0.19995 |
| Rwork | 0.198 |
| R-free | 0.24337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hd7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.050 | 0.280 |
| Number of reflections | 131267 | |
| <I/σ(I)> | 15.1 | 3.5 |
| Completeness [%] | 99.4 | 97.4 |
| Redundancy | 7.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 280 | 0.05M MGCL2.6H2O, 0.1M HEPES PH 7.5, 30% PEG 550MME, 1 MICRO-MOLAR HYCANTHONE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 280K |
