3U8H
Functionally selective inhibition of Group IIA phospholipase A2 reveals a role for vimentin in regulating arachidonic acid metabolism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 1-BM-C |
Synchrotron site | APS |
Beamline | 1-BM-C |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2006-10-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.90020 |
Spacegroup name | P 31 |
Unit cell lengths | 74.792, 74.792, 49.926 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.480 - 2.300 |
R-factor | 0.1937 |
Rwork | 0.192 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.103 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.932 |
High resolution limit [Å] | 2.300 |
Number of reflections | 13593 |
Completeness [%] | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1 M Tris HCl pH 7.4, 10 mM CaCl2, 0.5 mM Beta-octyl glucoside and 4 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |