3U88
Crystal structure of human menin in complex with MLL1 and LEDGF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 187.990, 187.990, 238.417 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.650 - 3.000 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.093 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.113 |
| <I/σ(I)> | 22.9 |
| Completeness [%] | 99.9 |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | 1.6 M ammonium sulfate, pH 7.0, vapor diffusion, hanging drop, temperature 277K |
