3U82
Binding of herpes simplex virus glycoprotein D to nectin-1 exploits host cell adhesion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97916 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.532, 169.043, 183.316 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.378 - 3.164 |
R-factor | 0.3223 |
Rwork | 0.321 |
R-free | 0.34430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c36 3alp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.343 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.164 | 3.200 |
Rmerge | 0.102 | 0.504 |
Number of reflections | 13572 | |
<I/σ(I)> | 14.4 | 1.7 |
Completeness [%] | 94.9 | 82.7 |
Redundancy | 4.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 20% PEG 1000, 0.1M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dehydrate, pH 5.5 , VAPOR DIFFUSION, HANGING DROP, temperature 277K |