3U7Y

Structure of NIH45-46 Fab in complex with gp120 of 93TH057 HIV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	69.130, 70.500, 217.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.050 - 2.448
R-factor	0.2095
Rwork	0.207
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3U7W AND 3NGB
RMSD bond length	0.004
RMSD bond angle	0.754
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_805))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.050	2.510
High resolution limit [Å]	2.448	2.448
Number of reflections	39062
Completeness [%]	98.6	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	12% isopropanol, 10% polyethylene glycol 10,000, 0.1 M sodium citrate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K