3U7Y
Structure of NIH45-46 Fab in complex with gp120 of 93TH057 HIV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.130, 70.500, 217.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.050 - 2.448 |
R-factor | 0.2095 |
Rwork | 0.207 |
R-free | 0.25560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3U7W AND 3NGB |
RMSD bond length | 0.004 |
RMSD bond angle | 0.754 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_805)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.050 | 2.510 |
High resolution limit [Å] | 2.448 | 2.448 |
Number of reflections | 39062 | |
Completeness [%] | 98.6 | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 12% isopropanol, 10% polyethylene glycol 10,000, 0.1 M sodium citrate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |