3U7D
Crystal structure of the KRIT1/CCM1 FERM domain in complex with the heart of glass (HEG1) cytoplasmic tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-18 |
| Detector | Mar/Rayonix 3x3 Mosaic 225 |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.050, 76.820, 79.180 |
| Unit cell angles | 90.00, 113.62, 90.00 |
Refinement procedure
| Resolution | 72.550 - 2.490 |
| R-factor | 0.23745 |
| Rwork | 0.234 |
| R-free | 0.30878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.746 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.550 | 2.620 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.058 | 0.312 |
| Number of reflections | 26420 | |
| <I/σ(I)> | 5.3 | 2.3 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 10% PEG 4000 and 100mM Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
