3U1Q
Crystal Structure of M. tuberculosis LD-transpeptidase type 2 with 2-Mercaptoethanol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-01 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 117.689, 121.013, 122.799 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.520 - 2.400 |
R-factor | 0.1884 |
Rwork | 0.185 |
R-free | 0.24420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 1.905 |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.520 |
High resolution limit [Å] | 2.400 |
Number of reflections | 34413 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.5 | 298 | 0.1 M Hepes (pH 7.5), 1 M succinic Acid, 1% (w/v) PEG MME 2000, hanging drop, temperature 298K |