3U0W
AD related murine antibody Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95466 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.047, 81.292, 108.474 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.390 - 2.000 |
| R-factor | 0.19145 |
| Rwork | 0.189 |
| R-free | 0.23543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qqn |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.004 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.390 |
| High resolution limit [Å] | 1.997 |
| Number of reflections | 39134 |
| Completeness [%] | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM citric acid, 20%(v/v) 2-propanol, 20%(w/v) PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
