3U0V
Crystal Structure Analysis of human LYPLAL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.782, 60.778, 76.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.730 - 1.720 |
| R-factor | 0.1753 |
| Rwork | 0.173 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fj2 |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.578 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.730 | 1.760 | |
| High resolution limit [Å] | 1.720 | 7.690 | 1.720 |
| Rmerge | 0.057 | 0.026 | 0.346 |
| Rmeas | 0.029 | 0.413 | |
| Number of reflections | 23960 | 293 | 1656 |
| <I/σ(I)> | 17.54 | 49.99 | 3.1 |
| Completeness [%] | 98.4 | 91 | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% w/v PEG 3350, 0.1M Bis-Tris pH 5.5, vapor diffusion, sitting drop, temperature 293K |
