3U0G
Crystal structure of branched-chain amino acid aminotransferase from burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97650 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 106.970, 139.270, 290.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.22964 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wrv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.762 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.076 |
| Number of reflections | 126128 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 20% PEG 8000, 100MM CHES, PROTEIN CONCENTRATION 23.1 MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, pH 9.50 |
