3TZH
Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(F187A) from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 63.341, 380.354, 63.212 |
Unit cell angles | 90.00, 119.86, 90.00 |
Refinement procedure
Resolution | 27.000 - 2.100 |
R-factor | 0.1587 |
Rwork | 0.157 |
R-free | 0.19110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.462 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 27.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.680 | 2.100 |
Rmerge | 0.046 | 0.023 | 0.111 |
Number of reflections | 73135 | ||
<I/σ(I)> | 13.8 | ||
Completeness [%] | 98.0 | 97.1 | 88.7 |
Redundancy | 2.9 | 3.2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9 | 0.1M Tris, 2.3M Ammonium Sulfate, pH 9.0, vapor diffusion |