3TZH
Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(F187A) from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.341, 380.354, 63.212 |
| Unit cell angles | 90.00, 119.86, 90.00 |
Refinement procedure
| Resolution | 27.000 - 2.100 |
| R-factor | 0.1587 |
| Rwork | 0.157 |
| R-free | 0.19110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.462 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 27.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.680 | 2.100 |
| Rmerge | 0.046 | 0.023 | 0.111 |
| Number of reflections | 73135 | ||
| <I/σ(I)> | 13.8 | ||
| Completeness [%] | 98.0 | 97.1 | 88.7 |
| Redundancy | 2.9 | 3.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 0.1M Tris, 2.3M Ammonium Sulfate, pH 9.0, vapor diffusion |
