3TXX
Crystal structure of putrescine transcarbamylase from Enterococcus faecalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 291 |
Detector technology | CCD |
Collection date | 2006-05-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 273.856, 87.791, 241.299 |
Unit cell angles | 90.00, 114.08, 90.00 |
Refinement procedure
Resolution | 48.170 - 3.200 |
R-factor | 0.1905 |
Rwork | 0.190 |
R-free | 0.23430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.269 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
Rmerge | 0.120 | 0.047 | 0.468 |
Number of reflections | 85439 | ||
<I/σ(I)> | 6.4 | ||
Completeness [%] | 98.5 | 99.7 | 87.4 |
Redundancy | 3.6 | 3.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 200 mM Magnesium sulfate, 15-17% PEG 3350 and 100 mM Bis-Tris pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |