3TWK
Crystal structure of arabidopsis thaliana FPG
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97930 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 93.054, 93.054, 157.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.222 |
R-free | 0.27320 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.303 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.090 |
Number of reflections | 31529 |
<I/σ(I)> | 22.6 |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 285 | 24% (w/v) PEG 3350, 175 mM MgCl2, and 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 285 | 24% (w/v) PEG 3350, 175 mM MgCl2, and 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 285 | 24% (w/v) PEG 3350, 175 mM MgCl2, and 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |