3TVW
Crystal Structure of the humanized carboxyltransferase domain of yeast Acetyl-coA caroxylase in complex with compound 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-BM |
Synchrotron site | APS |
Beamline | 17-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-29 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 246.964, 122.858, 146.073 |
Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
Resolution | 46.960 - 2.800 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.20530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w2x |
RMSD bond length | 0.010 |
RMSD bond angle | 1.180 |
Refinement software | BUSTER-TNT (BUSTER 2.9.3) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 106614 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 298 | 100 mM NaCitrate, 12%(w/v) PEG8000, 150 mM LiSO4, 7.5% (v/v) glycerol, pH 5.5, vapor diffusion, temperature 298K |