3TVR
Crystal Structure of Streptomyces coelicolor Polyketide Aromatase/Cyclase whiE-ORFVI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-3 |
| Synchrotron site | SSRL |
| Beamline | BL9-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.702, 42.060, 47.999 |
| Unit cell angles | 90.00, 91.89, 90.00 |
Refinement procedure
| Resolution | 34.390 - 1.800 |
| R-factor | 0.17503 |
| Rwork | 0.170 |
| R-free | 0.21414 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rer |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.964 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 17903 | |
| Completeness [%] | 99.7 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 277 | 0.1M Bis-Tris, 0.2M magnesium chloride, 28% PEG 3350, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
