3TVM
Structure of the mouse CD1d-SMC124-iNKT TCR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.970, 150.570, 101.960 |
| Unit cell angles | 90.00, 96.35, 90.00 |
Refinement procedure
| Resolution | 75.290 - 2.800 |
| R-factor | 0.22647 |
| Rwork | 0.224 |
| R-free | 0.27272 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2Q7Y for CD1d 3HE6 for TCR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.921 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.290 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.094 | 0.577 |
| Number of reflections | 58305 | |
| <I/σ(I)> | 10.9 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 100mM trisodium citrate pH5.6, 10% PEG3350, 2% tacsimate pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
