3TVK
Diguanylate cyclase domain of DgcZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 62 |
| Unit cell lengths | 79.623, 79.623, 51.207 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w25 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.281 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.210 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | 0.346 |
| Number of reflections | 17292 | |
| <I/σ(I)> | 24.1 | 7.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.5 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 100mM Tris, 22.5% PEG 4000, pH 8.5, vapor diffusion, hanging drop, temperature 293K |
