3TSO
Structure of the cancer associated Rab25 protein in complex with FIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.310, 64.210, 65.940 |
| Unit cell angles | 113.25, 107.24, 99.46 |
Refinement procedure
| Resolution | 39.180 - 1.800 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.969 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.411 | |
| Number of reflections | 50863 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 93.3 | 78.1 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 10% PEG8000, 0.1M Na/K phosphate pH 6.2, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
