3TPM
Crystal structure of MAL RPEL domain in complex with importin-alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-27 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.658, 90.116, 98.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.000 - 2.100 |
R-factor | 0.1681 |
Rwork | 0.166 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ial |
RMSD bond length | 0.022 |
RMSD bond angle | 2.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.340 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 41177 | |
Completeness [%] | 99.0 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.4 | 293 | 50mM Tris-HCl, 1.4M Li2SO4, pH 7.4, VAPOR DIFFUSION, temperature 293K |