3TPK
Crystal structure of the oligomer-specific KW1 antibody fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.921, 38.659, 94.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.870 - 1.300 |
| R-factor | 0.143 |
| Rwork | 0.141 |
| R-free | 0.17610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ln9 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.046 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.240 |
| High resolution limit [Å] | 1.210 | 5.410 | 1.210 |
| Rmerge | 0.031 | 0.016 | 0.383 |
| Number of reflections | 31606 | 460 | 1207 |
| <I/σ(I)> | 19.86 | 57.09 | 1.85 |
| Completeness [%] | 89.3 | 97.5 | 46.8 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 288 | 200 mM Magnesium Formate, 20%(w/v)PEG 3350, Additive: Benzamidine Hydrochloride 1M , pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
