3TOQ
Acylphosphatase with mesophilic surface and thermophilic core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.100, 78.100, 65.577 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.161 - 2.000 |
| R-factor | 0.1714 |
| Rwork | 0.167 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.918 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.057 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 10063 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 289 | 1.4 M Sodium Potassium, Phosphate pH 8.2 (Hampton Research, Index Screen B7 condition), 289 K , VAPOR DIFFUSION, SITTING DROP |
