3TO8
Crystal structure of the two C-terminal RRM domains of heterogeneous nuclear ribonucleoprotein L (hnRNP L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.569, 56.545, 88.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.690 - 1.820 |
| R-factor | 0.19239 |
| Rwork | 0.190 |
| R-free | 0.24274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2adc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.241 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.074 | 0.438 |
| Number of reflections | 17147 | |
| <I/σ(I)> | 23.816 | 4.75 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 30% v/v Pentaerythritol ethoxylate (15/4 EO/OH), 0.05M Ammonium sulfate, 0.05M BIS-TRIS pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
