3TM6
Crystal structure of the beta-2 microglobulin DIMC50 disulphide-linked homodimer mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.93950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.102, 56.272, 96.374 |
Unit cell angles | 90.00, 115.67, 90.00 |
Refinement procedure
Resolution | 29.390 - 2.700 |
R-factor | 0.2353 |
Rwork | 0.234 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z9t |
RMSD bond length | 0.007 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.390 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 23049 | |
Completeness [%] | 95.6 | 97.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | imidazole-malate 0.2 M, pH 5.5, PEG 600 24%, VAPOR DIFFUSION, SITTING DROP, temperature 293K |