3TM6
Crystal structure of the beta-2 microglobulin DIMC50 disulphide-linked homodimer mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.102, 56.272, 96.374 |
| Unit cell angles | 90.00, 115.67, 90.00 |
Refinement procedure
| Resolution | 29.390 - 2.700 |
| R-factor | 0.2353 |
| Rwork | 0.234 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z9t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 23049 | |
| Completeness [%] | 95.6 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | imidazole-malate 0.2 M, pH 5.5, PEG 600 24%, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
