3TJZ
Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 163.579, 163.579, 145.188 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.900 |
R-factor | 0.2087 |
Rwork | 0.206 |
R-free | 0.26020 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.285 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.061 | 0.432 |
Number of reflections | 83327 | |
<I/σ(I)> | 32.1 | 4.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 9% PEG 400, 0.1 M MES-NaOH pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |