3TJE

Crystal structure of Fas receptor extracellular domain in complex with Fab E09

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2007-09-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9000
Spacegroup name	C 2 2 21
Unit cell lengths	89.490, 166.400, 110.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.090 - 1.930
R-factor	0.2058
Rwork	0.204
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2H9G 1gig
RMSD bond length	0.009
RMSD bond angle	1.089
Data scaling software	XSCALE
Phasing software	PHASER (1.3.3)
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.848		1.980
High resolution limit [Å]	1.930	8.630	1.930
Rmerge	0.045	0.032	0.439
Number of reflections	61296	766	4548
<I/σ(I)>	22.51	51.81	4.73
Completeness [%]	98.3	97.5	99.6
Redundancy	6.5		6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	20% PEG4000, 5 mM CdCl2, 100 mM Tris/HOAc, pH 8.5, vapor diffusion, sitting drop, temperature 277K