3TJ3

Structure of importin a5 bound to the N-terminus of Nup50

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-20
Detector	ADSC QUANTUM 210
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	81.683, 98.291, 135.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.028 - 2.702
R-factor	0.217
Rwork	0.215
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jdq
RMSD bond length	0.005
RMSD bond angle	0.795
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.532	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	29294
<I/σ(I)>	26.86	2.15
Completeness [%]	96.8	94.1
Redundancy	3.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	24% PEG3350, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	24% PEG3350, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K