3TI0
Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to DNA and ddGTP-dC in Closed Conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97121 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.500, 108.920, 149.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.960 - 1.620 |
R-factor | 0.1879 |
Rwork | 0.187 |
R-free | 0.21500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2hvi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.221 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.710 |
High resolution limit [Å] | 1.620 | 4.840 | 1.620 |
Rmerge | 0.073 | 0.038 | 0.493 |
Number of reflections | 190278 | ||
<I/σ(I)> | 12.2 | 31.72 | 3.46 |
Completeness [%] | 98.3 | 95.5 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 45-50% saturated ammonium sulfate, 2.5% MPD, 10 mM magnesium sulfate, 100 mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |